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We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.

Original publication

DOI

10.1038/nmeth953

Type

Journal article

Journal

Nat Methods

Publication Date

11/2006

Volume

3

Pages

887 - 889

Keywords

Animals, Antibodies, Antigen-Antibody Reactions, Antigens, Camelus, Epitopes, Green Fluorescent Proteins, Immunoglobulin Heavy Chains, Mitosis, S Phase