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Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology.

Original publication

DOI

10.1038/srep15915

Type

Journal article

Journal

Sci Rep

Publication Date

03/11/2015

Volume

5

Keywords

Algorithms, Calibration, Image Processing, Computer-Assisted, Imaging, Three-Dimensional, Lighting, Microscopy, Fluorescence, Reproducibility of Results