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Sas-6 and Ana2/STIL proteins are required for centriole duplication and the homo-oligomerisation properties of Sas-6 help establish the ninefold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved, but they all contain a predicted Central Coiled-Coil Domain (CCCD). Here we show that the Drosophila Ana2 CCCD forms a tetramer, and we solve its structure to 0.8 Å, revealing that it adopts an unusual parallel-coil topology. We also solve the structure of the Drosophila Sas-6 N-terminal domain to 2.9 Å revealing that it forms higher-order oligomers through canonical interactions. Point mutations that perturb Sas-6 or Ana2 homo-oligomerisation in vitro strongly perturb centriole assembly in vivo. Thus, efficient centriole duplication in flies requires the homo-oligomerisation of both Sas-6 and Ana2, and the Ana2 CCCD tetramer structure provides important information on how these proteins might cooperate to form a cartwheel structure.

Original publication

DOI

10.7554/eLife.07236

Type

Journal article

Journal

Elife

Publication Date

23/05/2015

Volume

4

Keywords

Ana2, D. melanogaster, SAS-6, STIL, biophysics, cell biology, centriole, centrosome, human, structural biology, ultra-high resolution structure, Animals, Cell Cycle Proteins, Centrioles, Crystallography, X-Ray, DNA Mutational Analysis, Drosophila Proteins, Drosophila melanogaster, Microtubule-Associated Proteins, Point Mutation, Protein Conformation, Protein Multimerization