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Anthropogenic activities have resulted in cyanide (CN) contamination of both soil and water in many areas of the globe. While plants possess a detoxification pathway that serves to degrade endogenously generated CN, this system is readily overwhelmed, limiting the use of plants in bioremediation. Genetic engineering of additional CN degradation pathways in plants is one potential strategy to increase their tolerance to CN. Here we show that heterologous expression of microbial nitrilase enzymes targeted to the mitochondria increases CN tolerance in Arabidopsis. Root length in seedlings expressing either a CN dihydratase from Bacillus pumilis or a CN hydratase from Neurospora crassa was increased by 45% relative in wild-type plants in the presence of 50 μm KCN. We also demonstrate that in contrast to its strong inhibitory effects on seedling establishment, seed germination of the Col-0 ecotype of Arabidopsis is unaffected by CN.

Original publication

DOI

10.1111/plb.12323

Type

Journal article

Journal

Plant Biol (Stuttg)

Publication Date

07/2015

Volume

17

Pages

922 - 926

Keywords

Arabidopsis, bioremediation, cyanide, germination, mitochondria, nitrilase, Aminohydrolases, Arabidopsis, Bacillus, Bacterial Proteins, Cyanides, Fungal Proteins, Gene Expression, Germination, Hydro-Lyases, Hydrolases, Mitochondria, Neurospora crassa, Seedlings, Transgenes