Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Epidermal cells of the Arabidopsis thaliana root are derived from a ring of 16 or so initials (stem cells) that are located just below the quiescent centre of four central cells. Derivatives of these initials divide transversely in the meristematic region of the root to produce files of cells that subsequently elongate and differentiate. There are two kinds of cell file in the epidermis, hair cells and non-hair cells, that are organised in an ordered pattern and become distinguishable within a few divisions of the initials. It is likely that local intercellular communication plays an important role in the early establishment of this pattern, enabling cells to register their position and fate. We have therefore examined the extent to which cells in the root apex are symplastically coupled through plasmodesmata. Using a variety of dye loading methods we show that the undifferentiated cells are dye-coupled i.e. cells in the meristem and the elongation zone are symplastically connected, but that cells gradually become symplastically isolated as the cells differentiate. By the time that visible hair outgrowth is observed the cells of the epidermis are symplastically isolated. This resembles the pattern of gap-junction connectivity observed in many animal systems where cells are coupled at the stage that cell patterns become established, but become progressively more isolated as they differentiate.


Journal article



Publication Date





3247 - 3255