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Squalene epoxidase enzymes catalyse the conversion of squalene into 2,3-oxidosqualene, the precursor of cyclic triterpenoids. Here we report that the Arabidopsis drought hypersensitive/squalene epoxidase 1-5 (dry2/sqe1-5) mutant, identified by its extreme hypersensitivity to drought stress, has altered stomatal responses and root defects because of a point mutation in the SQUALENE EPOXIDASE 1 (SQE1) gene. GC-MS analysis indicated that the dry2/sqe1-5 mutant has altered sterol composition in roots but wild-type sterol composition in shoots, indicating an essential role for SQE1 in root sterol biosynthesis. Importantly, the stomatal and root defects of the dry2/sqe1-5 mutant are associated with altered production of reactive oxygen species. As RHD2 NADPH oxidase is de-localized in dry2/sqe1-5 root hairs, we propose that sterols play an essential role in the localization of NADPH oxidases required for regulation of reactive oxygen species, stomatal responses and drought tolerance.

Original publication

DOI

10.1111/j.1365-313X.2009.03849.x

Type

Journal article

Journal

Plant J

Publication Date

07/2009

Volume

59

Pages

63 - 76

Keywords

Arabidopsis, Arabidopsis Proteins, DNA, Plant, Dehydration, Droughts, Gene Expression Regulation, Plant, Genetic Complementation Test, Mutation, NADPH Oxidases, Oligonucleotide Array Sequence Analysis, Plant Roots, Plant Stomata, Reactive Oxygen Species, Squalene Monooxygenase, Sterols, Stress, Physiological