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In yeast, many tandemly arranged genes show peak expression in different phases of the metabolic cycle (YMC) or in different carbon sources, indicative of regulation by a bi-modal switch, but it is not clear how these switches are controlled. Using native elongating transcript analysis (NET-seq), we show that transcription itself is a component of bi-modal switches, facilitating reciprocal expression in gene clusters. HMS2, encoding a growth-regulated transcription factor, switches between sense- or antisense-dominant states that also coordinate up- and down-regulation of transcription at neighbouring genes. Engineering HMS2 reveals alternative mono-, di- or tri-cistronic and antisense transcription units (TUs), using different promoter and terminator combinations, that underlie state-switching. Promoters or terminators are excluded from functional TUs by read-through transcriptional interference, while antisense TUs insulate downstream genes from interference. We propose that the balance of transcriptional insulation and interference at gene clusters facilitates gene expression switches during intracellular and extracellular environmental change.

Original publication

DOI

10.7554/eLife.03635

Type

Journal article

Journal

Elife

Publication Date

19/11/2014

Volume

3

Keywords

HMS2:BAT2, S. cerevisiae, chromosomes, computational biology, cycling transcripts, gene clusters, genes, systems biology, transcription insulation, transcription interference, yeast metabolic cycle, Carbon, Gene Expression Regulation, Fungal, Genes, Fungal, Genetic Loci, Metabolic Networks and Pathways, Multigene Family, RNA, Antisense, RNA, Messenger, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic