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1. Na+/H+ antiport activity was measured in peripheral blood polymorphonuclear and mononuclear cells of 12 healthy subjects by using an intracellular pH clamp technique to determine the external Na(+)-dependent H+ efflux rate in cells loaded with a pH-sensitive fluorescent dye, bis(carboxyethyl)carboxyfluorescein. The change in external Na+ concentrations for all pH measurements was similar in both cell types. 2. A significant difference between the two types of cells was found, the polymorphonuclear leucocytes having a higher Na+/H+ antiport activity than the lymphocytes. Cellular intrinsic buffering capacity measured in the absence of HCO3- was also higher in the polymorphonuclear cells than in the lymphocytes. 3. These differences may be associated with a difference in the role of the Na+/H+ exchanger in these two types of cells, although in vivo the presence of HCO3-/Cl- exchangers may also contribute to intracellular pH homoeostasis.


Journal article


Clin Sci (Lond)

Publication Date





95 - 99


Adult, Amiloride, Anti-Arrhythmia Agents, Buffers, Carrier Proteins, Female, Humans, Hydrogen, Hydrogen-Ion Concentration, In Vitro Techniques, Leukocytes, Lymphocytes, Male, Meglumine, Neutrophils, Sodium Chloride, Sodium-Hydrogen Exchangers