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Nicotinic acid adenine dinucleotide phosphate (NAADP), like the other major messengers for Ca²⁺ mobilization, is passively membrane-impermeant. Instead, a cell-permeant acetoxymethyl ester derivative of NAADP (NAADP-AM) can be synthesized as described here and used to study NAADP-mediated Ca²⁺ release.

Original publication

DOI

10.1101/pdb.prot076927

Type

Journal article

Journal

Cold Spring Harb Protoc

Publication Date

01/10/2014

Volume

2014

Keywords

Animals, Calcium, Calcium Signaling, Cells, Cultured, Humans, NADP, Permeability