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The PhoD family of extra-cytoplasmic phosphodiesterases are among the most commonly occurring bacterial phosphatases. The exemplars for this family are the PhoD protein of Bacillus subtilis and the phospholipase D of Streptomyces chromofuscus. We present the crystal structure of B. subtilis PhoD. PhoD is most closely related to purple acid phosphatases (PAPs) with both types of enzyme containing a tyrosinate-ligated Fe(3+) ion. However, the PhoD active site diverges from that found in PAPs and uses two Ca(2+) ions instead of the single extra Fe(2+), Mn(2+), or Zn(2+) ion present in PAPs. The PhoD crystals contain a phosphate molecule that coordinates all three active site metal ions and that is proposed to represent a product complex. A C-terminal helix lies over the active site and controls access to the catalytic center. The structure of PhoD defines a new phosphatase active site architecture based on Fe(3+) and Ca(2+) ions.

Original publication

DOI

10.1074/jbc.M114.604892

Type

Journal article

Journal

J Biol Chem

Publication Date

07/11/2014

Volume

289

Pages

30889 - 30899

Keywords

Bacterial Metabolism, Catalysis, Metalloenzyme, Phosphatase, Structural Biology, Amino Acid Sequence, Bacillus subtilis, Bacterial Proteins, Calcium, Catalytic Domain, Crystallography, X-Ray, Electron Spin Resonance Spectroscopy, Hydrogen-Ion Concentration, Iron, Ligands, Metals, Molecular Sequence Data, Phosphoric Diester Hydrolases, Phosphoric Monoester Hydrolases, Protein Structure, Secondary, Sequence Homology, Amino Acid, Tyrosine, Zinc