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The kinetochore is the macromolecular protein complex that directs chromosome segregation in eukaryotes. It has been widely assumed that the core kinetochore consists of proteins that are common to all eukaryotes. However, no conventional kinetochore components have been identified in any kinetoplastid genome, thus challenging this assumption of universality. Here, we report the identification of 19 kinetochore proteins (KKT1-19) in Trypanosoma brucei. The majority is conserved among kinetoplastids, but none of them has detectable homology to conventional kinetochore proteins. These proteins instead have a variety of features not found in conventional kinetochore proteins. We propose that kinetoplastids build kinetochores using a distinct set of proteins. These findings provide important insights into the longstanding problem of the position of the root of the eukaryotic tree of life.

Original publication

DOI

10.1016/j.cell.2014.01.049

Type

Journal article

Journal

Cell

Publication Date

13/03/2014

Volume

156

Pages

1247 - 1258

Keywords

Amino Acid Sequence, Chromosome Segregation, DNA, Kinetoplast, Kinetochores, Molecular Sequence Data, Protozoan Proteins, Sequence Alignment, Trypanosoma brucei brucei