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Laser Doppler flowmetry (LDF) is a method by which relative cerebral blood flow (CBF) of the cortex can be measured. Although the method is easy to employ, LDF only measures relative CBF, while absolute CBF cannot be quantified. LDF is useful for investigating CBF changes in a number of different applications including neurovascular and stroke research. This chapter will prepare the reader for rodent experiments using LDF with two preparations. The closed skull preparation can be used to monitor CBF with an intact skull, but in adult rats, thinning of the skull is required to obtain an accurate cortical CBF signal. The open skull preparation requires a craniotomy to expose the surface of the brain and the LDF probe is held close to the surface to measure cerebral perfusion.

Original publication

DOI

10.1007/978-1-4939-0320-7_20

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2014

Volume

1135

Pages

237 - 248

Keywords

Animals, Cerebral Cortex, Cerebrovascular Circulation, Laser-Doppler Flowmetry, Male, Rats, Rats, Wistar