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The analysis of stable isotope incorporation following feeding of (13)C-labeled precursors to plant tissues provides the constraints necessary for metabolic flux analysis. This protocol describes the use of one-dimensional (1)H and (13)C nuclear magnetic resonance spectroscopy for the quantification of (13)C enrichments and isotopomer abundances in mixtures of metabolites or hydrolyzed biomass components.

Type

Journal article

Journal

Methods in molecular biology (Clifton, N.J.)

Publication Date

09/07/2014

Volume

1090

Pages

73 - 86