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The analysis of stable isotope incorporation following feeding of (13)C-labeled precursors to plant tissues provides the constraints necessary for metabolic flux analysis. This protocol describes the use of one-dimensional (1)H and (13)C nuclear magnetic resonance spectroscopy for the quantification of (13)C enrichments and isotopomer abundances in mixtures of metabolites or hydrolyzed biomass components.

Original publication

DOI

10.1007/978-1-62703-688-7_5

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2014

Volume

1090

Pages

73 - 86

Keywords

Carbon Isotopes, Fourier Analysis, Magnetic Resonance Spectroscopy, Metabolic Flux Analysis, Plants, Staining and Labeling