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While steady-state (13)C metabolic flux analysis is a powerful method for deducing multiple fluxes in the central metabolic network of heterotrophic and mixotrophic plant tissues, it is also time-consuming and technically challenging. Key steps in the design and interpretation of steady-state (13)C labeling experiments are illustrated with a generic protocol based on applications to plant cell suspension cultures.

Original publication




Journal article


Methods Mol Biol

Publication Date





53 - 72


Algorithms, Arabidopsis, Carbohydrate Metabolism, Carbon Isotopes, Cell Culture Techniques, Cells, Cultured, Computer Simulation, Kinetics, Metabolic Flux Analysis, Metabolic Networks and Pathways, Models, Biological, Monte Carlo Method, Plant Cells, Staining and Labeling