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While steady-state (13)C metabolic flux analysis is a powerful method for deducing multiple fluxes in the central metabolic network of heterotrophic and mixotrophic plant tissues, it is also time-consuming and technically challenging. Key steps in the design and interpretation of steady-state (13)C labeling experiments are illustrated with a generic protocol based on applications to plant cell suspension cultures.

Original publication

DOI

10.1007/978-1-62703-688-7_4

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2014

Volume

1090

Pages

53 - 72

Keywords

Algorithms, Arabidopsis, Carbohydrate Metabolism, Carbon Isotopes, Cell Culture Techniques, Cells, Cultured, Computer Simulation, Kinetics, Metabolic Flux Analysis, Metabolic Networks and Pathways, Models, Biological, Monte Carlo Method, Plant Cells, Staining and Labeling