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Signaling through the T cell receptor (TCR) initiates adaptive immunity and its perturbation may results in autoimmunity. The plasma membrane scaffolding protein LAT acts as a central organizer of the TCR signaling machinery to activate many functional pathways. LAT-deficient mice develop an autoimmune syndrome but the mechanism of this pathology is unknown. In this work we have compared global dynamics of TCR signaling by MS-based quantitative phosphoproteomics in LAT-sufficient and LAT-defective Jurkat T cells. Surprisingly, we found that many TCR-induced phosphorylation events persist in the absence of LAT, despite ERK and PLCγ1 phosphorylation being repressed. Most importantly, the absence of LAT resulted in augmented and persistent tyrosine phosphorylation of CD3ζ and ZAP70. This indicates that LAT signaling hub is also implicated in negative feedback signals to modulate upstream phosphorylation events. Phosphorylation kinetics data resulting from this investigation is documented in a database (phosphoTCR) accessible online. The MS data have been deposited to the ProteomeXchange with identifier PXD000341.

Original publication

DOI

10.1371/journal.pone.0077423

Type

Journal article

Journal

PLoS One

Publication Date

2013

Volume

8

Keywords

Adaptor Proteins, Signal Transducing, Antigens, CD3, Chromatography, Liquid, Databases, Protein, Feedback, Physiological, Gene Expression Regulation, Humans, Jurkat Cells, Mass Spectrometry, Membrane Proteins, Phospholipase C gamma, Phosphoproteins, Phosphorylation, Protein Interaction Mapping, Receptors, Antigen, T-Cell, Signal Transduction, Tyrosine, ZAP-70 Protein-Tyrosine Kinase