Observations on the cytoplasmic and vacuolar orthophosphate pools in leaf tissues using in vivo<sup>31</sup>P-NMR spectroscopy
Loughman BC., Ratcliffe RG., Southon TE.
A full understanding of the central role of Piin photosynthesis requires information on the size of the endogenous Pipools and the extent to which they interact with each other under different physiological conditions. In vivo31P-NMR spectroscopy has the potential to assist in this objective but the early applications of this technique to leaf tissues were disappointing. Here we report31P-NMR data from maize and tomato leaf discs that: (i) cast doubt on earlier estimates of the cytoplasmic orthophosphate pool size; and (ii) prove that exogenously supplied D-mannose can reduce the cytoplasmic Pilevel in some circumstances. © 1989.