Observations on the cytoplasmic and vacuolar orthophosphate pools in leaf tissues using in vivo 31P-NMR spectroscopy
Loughman BC., Ratcliffe RG., Southon TE.
A full understanding of the central role of P i in photosynthesis requires information on the size of the endogenous P i pools and the extent to which they interact with each other under different physiological conditions. In vivo 31 P-NMR spectroscopy has the potential to assist in this objective but the early applications of this technique to leaf tissues were disappointing. Here we report 31 P-NMR data from maize and tomato leaf discs that: (i) cast doubt on earlier estimates of the cytoplasmic orthophosphate pool size; and (ii) prove that exogenously supplied D-mannose can reduce the cytoplasmic P i level in some circumstances. © 1989.