Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

A pulsed EPR saturation-recovery method has been used to measure spin-lattice relaxation times (T 1) for aminoxyl spin labels in cardiolipin bilayers, with and without cytochrome c. The relaxation time for each spin label was determined at various positions in the bilayer, which included the bilayer surface and three positions within the hydrophobia interior in the membrane, as well as a position close to the glycerol backbone of phospholipids in the bilayer. A dynamic profile for the hydrocarbon chains in bilayers was found in agreement with results from other techniques. On addition of cytochrome c to cardiolipin bilayers, changes in the spin-lattice relaxation time and order parameter were observed around the unsaturated region in the cardiolipin acyl chains. Supported by complementary lineshape analysis, these effects were interpreted as changes in the molecular dynamics around the double bonds in the acyl chains of cardiolipin bilayers, induced upon the binding of cytochrome c.


Journal article


Journal of the Chemical Society, Perkin Transactions 2

Publication Date



2113 - 2117