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Organophosphate and -phosphonates and their thio derivatives are often used in agroindustry as herbicides and insecticides, but their potential off-targets in the plant are poorly investigated. Here, we use competitive activity-based protein profiling (ABPP) of serine hydrolases (SHs) to detect targets of these agrochemicals and other compounds in Arabidopsis thaliana. Using broad-range and specific probes, and by overexpression of various SHs in planta, we are able to confirm eight SH-compound interactions, including selective inhibition of carboxylesterase CXE12, prolyloligopeptidase, methylesterase MES2 and tripeptidyl peptidase TPP2. These observations can be used for the design of novel probes and selective inhibitors and may help to assess physiological effects of agrochemicals on crop plants.

Original publication

DOI

10.1016/j.bmc.2011.06.040

Type

Journal article

Journal

Bioorg Med Chem

Publication Date

15/01/2012

Volume

20

Pages

597 - 600

Keywords

Agrochemicals, Arabidopsis, Herbicides, Insecticides, Organophosphates, Plant Proteins, Proteomics, Serine Proteases, Serine Proteinase Inhibitors