Sulfonyl fluoride analogues as activity-based probes for serine proteases.
Shannon DA., Gu C., McLaughlin CJ., Kaiser M., van der Hoorn RA., Weerapana E.
Enriched with fluoride: To expand on the available tools to interrogate proteases, we explored sulfonyl fluorides as activity-based probes. An alkyne-tagged sulfonyl fluoride covalently modifies members of the S1 family of serine proteases. By applying click chemistry, avidin enrichment and mass spectrometry, we can enrich and identify active endogenous serine proteases from a complex proteome.