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Enriched with fluoride: To expand on the available tools to interrogate proteases, we explored sulfonyl fluorides as activity-based probes. An alkyne-tagged sulfonyl fluoride covalently modifies members of the S1 family of serine proteases. By applying click chemistry, avidin enrichment and mass spectrometry, we can enrich and identify active endogenous serine proteases from a complex proteome.

Original publication

DOI

10.1002/cbic.201200531

Type

Journal article

Journal

Chembiochem

Publication Date

05/11/2012

Volume

13

Pages

2327 - 2330

Keywords

Molecular Probes, Molecular Structure, Serine Proteases, Serine Proteinase Inhibitors, Structure-Activity Relationship, Sulfinic Acids