Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Procedures have been described which are suitable for experiments utilizing a microculture system whereby one can easily study the function of single helper T cells. These are: (a) the elimination of the background anti-hapten response by preliminary anti-theta + guinea-pig complement complement treatment of the 'hapten-primed' population; (b) the removal of any contaminating hapten-specific B cells from the carrier-primed population by passage through nylon wool; (c) irradiation of long-term primed 'nylon wool' enriched helper cells tp prevent cell division; (d) the partition of individual helper cells by limiting dilution.

Type

Journal article

Journal

Immunology

Publication Date

09/1976

Volume

31

Pages

343 - 352

Keywords

Animals, Antibody-Producing Cells, B-Lymphocytes, Dose-Response Relationship, Immunologic, Haptens, Hemolytic Plaque Technique, Leukocyte Count, Mice, T-Lymphocytes