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There is a need for vaccines that can protect broadly across all influenza A strains. We have produced a pseudotyped influenza virus based on suppression of the A/PR/8/34 hemagglutinin signal sequence (S-FLU) that can infect cells and express the viral core proteins and neuraminidase but cannot replicate. We show that when given by inhalation to mice, S-FLU is nonpathogenic but generates a vigorous T cell response in the lung associated with markedly reduced viral titers and weight loss after challenge with H1 and H3 influenza viruses. These properties of S-FLU suggest that it may have potential as a broadly protective A virus vaccine, particularly in the setting of a threatened pandemic before matched subunit vaccines become available.

Original publication

DOI

10.1128/JVI.01820-12

Type

Journal article

Journal

J Virol

Publication Date

12/2012

Volume

86

Pages

13397 - 13406

Keywords

Administration, Inhalation, Animals, Antibodies, Viral, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Indirect, Hemagglutinin Glycoproteins, Influenza Virus, Influenza A virus, Influenza Vaccines, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Neutralization Tests, T-Lymphocytes