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Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the mitochondrial malate dehydrogenase gene in the antisense orientation and exhibiting reduced activity of this isoform of malate dehydrogenase show enhanced photosynthetic activity and aerial growth under atmospheric conditions (360 ppm CO2). In comparison to wild-type plants, carbon dioxide assimilation rates and total plant dry matter were up to 11% and 19% enhanced in the transgenics, when assessed on a whole-plant basis. Accumulation of carbohydrates and redox-related compounds such as ascorbate was also markedly elevated in the transgenics. Also increased in the transgenic plants was the capacity to use L-galactono-lactone, the terminal precursor of ascorbate biosynthesis, as a respiratory substrate. Experiments in which ascorbate was fed to isolated leaf discs also resulted in increased rates of photosynthesis providing strong indication for an ascorbate-mediated link between the energy-generating processes of respiration and photosynthesis. This report thus shows that the repression of this mitochondrially localized enzyme improves both carbon assimilation and aerial growth in a crop species.

Original publication

DOI

10.1104/pp.104.055566

Type

Journal article

Journal

Plant Physiol

Publication Date

02/2005

Volume

137

Pages

611 - 622

Keywords

Ascorbic Acid, Chloroplasts, DNA, Complementary, Electron Transport, Lycopersicon esculentum, Malate Dehydrogenase, Mitochondria, Molecular Sequence Data, Oxygen Consumption, Phenotype, Photosynthesis, Phylogeny, Plants, Genetically Modified