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A solid-phase enzyme-linked binding assay is described for screening monoclonal antibodies to cell surface antigens. E. coli beta-galactosidase was coupled to rabbit anti-rat Ig and used to detect the binding of rat monoclonal antibodies to cells which had been fixed to the wells of microtitre plates using a combination of poly-L-lysine and glutaraldehyde. This method was found to be advantageous for the large screening of monoclonal antibodies with a panel of cell types, and has been useful in the selection of antibodies which would be candidates for differentiation markers within the human and mouse haemopoietic systems.

Type

Journal article

Journal

J Immunol Methods

Publication Date

1981

Volume

44

Pages

125 - 133

Keywords

Animals, Antibodies, Monoclonal, Antigen-Antibody Reactions, Antigens, Surface, Cell Adhesion, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Glutaral, Immunoglobulins, Incubators, Rabbits, Radioimmunoassay, Rats, beta-Galactosidase