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For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new approaches have created unprecedented new possibilities to investigate the structure and function of cells.

Original publication

DOI

10.1083/jcb.201002018

Type

Journal article

Journal

J Cell Biol

Publication Date

26/07/2010

Volume

190

Pages

165 - 175

Keywords

Animals, Fluorescent Dyes, Light, Microscopy, Fluorescence