Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Reconstruction of a gene with its introns removed results in reduced levels of cytoplasmic mRNA. This is partly explained by introns promoting the export of mRNA through coupling splicing to nuclear export processes. However, we show here that splicing signals can have a direct role in enhancing gene transcription. Removal of promoter proximal splice signals from a mammalian gene or the excision of introns from two different yeast genes results in a marked reduction in levels of nascent transcription, based on both nuclear run-on and direct image analysis. This further establishes that mRNA processing and transcription are tightly coupled mechanisms.

Original publication

DOI

10.1101/gad.983602

Type

Journal article

Journal

Genes Dev

Publication Date

01/11/2002

Volume

16

Pages

2792 - 2799

Keywords

Genes, Fungal, Genes, Viral, HIV-1, Promoter Regions, Genetic, RNA Splice Sites, RNA, Messenger, Saccharomyces cerevisiae, Transcription, Genetic