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Evidence is presented here that cupric ions play a part in the natural spinning of Bombyx mori silk. Proton induced X-ray emission studies revealed that the copper content increased from the posterior part to the anterior part of silk gland, and then further increased in the silk fiber. Spectrophotometric analysis demonstrated that cupric ions formed coordination complexes with silk fibroin chains while Raman spectroscopy indicated that they induced a conformation transition from random coil/helix to beta-sheet. Taken together these findings indicate that copper could play a role in the natural spinning process in silkworms.

Original publication

DOI

10.1016/s0014-5793(03)01184-0

Type

Journal article

Journal

FEBS Lett

Publication Date

20/11/2003

Volume

554

Pages

337 - 341

Keywords

Animals, Bombyx, Copper, Fibroins, Insect Proteins, Neutral Red, Protein Structure, Secondary, Silk, Spectrometry, X-Ray Emission, Spectrum Analysis, Raman