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Neisseria meningitidis is an exclusively human pathogen that has evolved primarily to colonize the nasopharynx rather than to cause systemic disease. Colonization is the most frequent outcome following meningococcal infection and a prerequisite for invasive disease. The mechanism of colonization involves attachment of the organism to epithelial cells via bacterial type IV pili (Tfp), but subsequent events during colonization remain largely unknown. We analyzed 576 N. meningitidis mutants for their capacity to colonize human nasopharyngeal tissue in an organ culture model to identify bacterial genes required for colonization. Eight colonization-defective mutants were isolated. Two mutants were unable to express Tfp and were defective for adhesion to epithelial cells, which is likely to be the basis of their attenuation in nasopharyngeal tissue. Three other mutants are predicted to have lost previously uncharacterized surface molecules, while the remaining mutants have transposon insertions in genes of unknown function. We have identified novel meningococcal colonization factors, and this should provide insights into the survival of this important pathogen in its natural habitat.

Original publication

DOI

10.1128/IAI.00968-08

Type

Journal article

Journal

Infect Immun

Publication Date

01/2009

Volume

77

Pages

45 - 51

Keywords

Adhesins, Bacterial, Bacterial Adhesion, Bacterial Proteins, Epithelial Cells, Fimbriae, Bacterial, Humans, Mutagenesis, Insertional, Nasopharynx, Neisseria meningitidis, Organ Culture Techniques, Virulence Factors