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Farnesylated prelamin A accumulates when the final endoproteolytic maturation of the protein fails to occur and causes a dysmorphic nuclear phenotype; however, the morphology and mechanisms of biogenesis of these changes remain unclear. We show here that acute prelamin A accumulation after reduction in the activity of the ZMPSTE24 endoprotease by short interfering RNA knockdown, results in the generation of a complex nucleoplasmic reticulum that depends for its formation on the enzyme CTP:phosphocholine-cytidylyltransferase-α (CCT-α, also known as choline-phosphate cytidylyltransferase A). This structure can form during interphase, confirming that it is independent of mitosis and therefore not a consequence of disordered nuclear envelope assembly. Serial-section dual-axis electron tomography reveals that these invaginations can take two forms: one in which the inner nuclear membrane infolds alone with an inter membrane space interior, and the other in which an invagination of both nuclear membranes occurs, enclosing a cytoplasmic core. Both types of invagination can co-exist in one nucleus and both are frequently studded with nuclear pore complexes (NPC), which reduces NPC abundance on the nuclear surface.

Original publication

DOI

10.1242/jcs.091009

Type

Journal article

Journal

J Cell Sci

Publication Date

15/12/2011

Volume

124

Pages

4253 - 4266

Keywords

Animals, Cell Nucleus, Cells, Cultured, Choline-Phosphate Cytidylyltransferase, Lamin Type A, Lamin Type B, Membrane Proteins, Metalloendopeptidases, Mice, Mitosis, Nuclear Envelope, Nuclear Pore, Nuclear Proteins, Prenylation, Protein Precursors