Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

We have recently established a system for purifying minichromosomes in a native state from Saccharomyces cerevisiae. This system is extremely efficient, and a single-step purification yields samples with sufficient purity and quantity for mass spectrometry (MS) analysis of histones and non-histone proteins tightly associated with the minichromosome. The templates can also be used in various biochemical assays in vitro, such as transcription and recombination, and could be suitable for EM or other biophysical studies.

Original publication




Journal article


Methods Mol Biol

Publication Date





115 - 123


Antibodies, Fungal, Chromosome Mapping, Chromosomes, Fungal, Electrophoresis, Polyacrylamide Gel, Magnetics, Microspheres, Molecular Biology, Plasmids, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins