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Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3-XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A-dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.

Original publication

DOI

10.1083/jcb.200503023

Type

Journal article

Journal

J Cell Biol

Publication Date

26/09/2005

Volume

170

Pages

1047 - 1055

Keywords

Animals, Aurora Kinases, Cell Cycle Proteins, Cell Extracts, Centrosome, Kinesin, Microtubule-Associated Proteins, Microtubules, Mitosis, Oocytes, Phosphorylation, Protein Kinases, Protein-Serine-Threonine Kinases, Transcription Factors, Xenopus Proteins, Xenopus laevis