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A panel of 86 different Candida albicans isolates was subjected to multilocus sequence typing (MLST) in two laboratories to obtain sequence data for 10 published housekeeping gene fragments. Analysis of data for all possible combinations of five, six, seven, eight, and nine of the fragments showed that a set comprising the fragments AAT1a, ACC1, ADP1, MPIb, SYA1, VPS13, and ZWF1b was the smallest that yielded 86 unique diploid sequence types for the 86 isolates. This set is recommended for future MLST with C. albicans.

Type

Journal article

Journal

J Clin Microbiol

Publication Date

11/2003

Volume

41

Pages

5265 - 5266

Keywords

Candida albicans, Consensus Sequence, DNA, Fungal, Diploidy, Genes, Fungal, Genetic Techniques