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Transcriptional regulation in higher eukaryotes frequently involves long-range interactions, up to tens of hundreds of kilobases away, of a number of cis-acting regulatory DNA elements. Using the chromosome conformation capture technique we have analyzed the expression of a small 2.5-kb gene, CD68, in different human cell types and show for the first time that short-range interactions may also be critical. In human monocytes, which produce high levels of CD68 mRNA, the gene is characterized by intramolecular ligations between the promoter and the 3' intervening region. In cells that poorly express the gene a change in architecture is apparent whereby the promoter preferentially associates with the terminator region only. Furthermore, alterations in CD68 gene structure are associated with failings in mRNA splicing and changes with the phosphorylation status of RNA Pol II across the gene. We propose that short-range intrachromosomal interactions may form the basis of coordinated control of monocyte-specific gene regulation.

Original publication

DOI

10.1016/j.ygeno.2007.04.010

Type

Journal article

Journal

Genomics

Publication Date

09/2007

Volume

90

Pages

407 - 415

Keywords

Antigens, CD, Antigens, Differentiation, Myelomonocytic, Cell Line, Cell Line, Tumor, Chromatin, Chromatin Immunoprecipitation, DNA Polymerase II, Gene Expression Regulation, HL-60 Cells, Humans, Jurkat Cells, Models, Genetic, Myeloid Cells, Phosphorylation, RNA, Messenger, Transcription, Genetic