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Macrophage scavenger receptors (MSR) are implicated in the development of atherosclerosis and amyloid b-protein deposition in Alzheimer's disease. However, histopathological studies of MSR expression in human tissues have been hampered by a lack of specific antibodies. Using MSR-deficient mice, we successfully raised a novel monoclonal antibody against human MSR together with high-titer antisera. These antibodies specifically recognized human tissue macrophages and human MSR protein purified from differentiated THP1 cells. In normal brain, MSR staining was mainly distributed to the perivascular cells, which correspond to Mato's fluorescent granular perithelial cells (Mato cells). In the lesions of ischemia and Alzheimer's disease, a subset of microglia stained positive for MSR. These novel antibodies are useful tools for analysis of MSR expression in human tissues.

Original publication

DOI

10.1006/bbrc.1998.8120

Type

Journal article

Journal

Biochem Biophys Res Commun

Publication Date

28/04/1998

Volume

245

Pages

734 - 740

Keywords

Alzheimer Disease, Animals, Antibodies, Monoclonal, Brain, Cell Adhesion Molecules, Cell Line, Enzyme-Linked Immunosorbent Assay, Humans, Immunohistochemistry, Ischemia, Mice, Microglia, Receptors, Immunologic, Receptors, Scavenger