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The translocation of the microtubule-organizing center (MTOC), its associated signaling complex, and the secretory apparatus is the most characteristic early event that involves the tubulin cytoskeleton of T or NK cells after their interaction with APC or target cells. Our results show that Fyn kinase activity is essential for MTOC reorientation in an Ag-dependent system. Moreover, T cells from Fyn-deficient mice are unable to rearrange their tubulin cytoskeleton in response to anti-CD3-coated beads. Analysis of conjugates of T cells from transgenic OT-I mice with dendritic cells revealed that an antagonist peptide induces translocation of the MTOC, and that this process is impaired in T cells from Fyn(-/-) OT-I mice. In addition, Fyn deficiency significantly affects the MTOC relocation mediated by agonist peptide stimulation. These results reveal Fyn to be a key regulator of tubulin cytoskeleton reorganization in T cells.

Type

Journal article

Journal

J Immunol

Publication Date

01/04/2006

Volume

176

Pages

4201 - 4207

Keywords

Adaptor Proteins, Signal Transducing, Animals, Cell Line, Cytoskeleton, Enzyme Activation, Humans, Mice, Mice, Knockout, Peptide Fragments, Proto-Oncogene Proteins c-fyn, Receptors, Antigen, T-Cell, T-Lymphocytes, Tubulin