Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

In situ Proximity Ligation Assay (PLA ) can be used to detect the close proximity (less than ~40 nm) of two biological molecules of interest in cells. Here we report the application of this method for the specific detection of R-loop interacting proteins and RNA modifications in close proximity to R-loops in non-damage and ionizing radiation (IR) induced DNA damage conditions.

Original publication

DOI

10.1007/978-1-0716-2477-7_19

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2022

Volume

2528

Pages

289 - 303

Keywords

DNA Damage, Double Strand breaks, Proximity Ligation Assay, R-loops, RNA modification, RNA:DNA hybrids