Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The effect of a cryoprotectant (glycerol) upon the mosaicity of flash-frozen T-state glycogen phosphorylase b crystals has been investigated. Glycerol concentration in a buffered solution was varied between 0 and 70% v/v. The results show that there is a well defined optimum in glycerol concentration giving lowest mosaicity, maximum resolution and no ice formation. The results also demonstrate how flash-freezing conditions can be systematically determined in-house using a minimum number of crystals and before using synchrotron time.

Original publication

DOI

10.1107/s0021889894008629

Type

Journal article

Journal

Journal of Applied Crystallography

Publication Date

01/01/1994

Volume

27

Pages

1070 - 1074