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The kinetics of bond formation between spherical beads coated with CD48 and CD2-derivatized surfaces was studied with a flow chamber. For a given shear rate, the binding frequency was exquisitively sensitive to the particle velocity. Flow equations were used to derive the particle-to-surface distance from the velocity, thus yielding a relationship between this distance and the binding rate. Numerical values of the binding site densities allowed absolute determination of the rate of association between two individual molecules as a function of the distance between attachment points. In our model, this rate was about 0.03 s-1 at 10 nm separation, and it was inversely proportional to the cube of the distance.

Type

Journal article

Journal

FEBS Lett

Publication Date

24/02/1997

Volume

403

Pages

239 - 244

Keywords

Animals, Antigens, CD, Antigens, CD2, CD48 Antigen, Cell Adhesion Molecules, Kinetics, Ligands, Microspheres, Protein Binding, Rats