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Radiation damage continues to present a problem to crystallographers using cryocooled protein crystals at third-generation synchrotrons. Free-radical scavengers have been suggested as a possible means of reducing the rate of this damage. The screening of a large number of potential radioprotectants was undertaken with an online microspectrophotometer using cystine and cysteine to model protein disulfide bonds and thiol groups, respectively. Oxidized alpha-lipoic acid was tested as a possible model disulfide bond. The evidence for the effectiveness of ascorbate as a radioprotectant was strengthened, and quinone, 2,2,6,6-tetramethyl-4-piperidone, and reduced dithiothreitol showed promise as radioprotectants.

Original publication

DOI

10.1107/S0909049506044177

Type

Journal article

Journal

J Synchrotron Radiat

Publication Date

01/2007

Volume

14

Pages

73 - 83

Keywords

Computer Simulation, Crystallography, X-Ray, Dose-Response Relationship, Radiation, Protein Conformation, Protein Denaturation, Proteins, Radiation Dosage, Radiation-Protective Agents, X-Rays