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Advances in cryocrystallographic techniques for macromolecular crystallography have been intimately intertwined with efforts to reduce the deleterious effects of X-ray damage inflicted during the collection of diffraction data. A brief overview of cryomethods and their rationale is given. This is followed by a summary of our current limited understanding of radiation damage in cryocooled crystals, investigations aimed at minimizing its effects and finally some developments which actually utilize it both for phasing and to extend structural knowledge.

Original publication

DOI

10.1107/S0907444905034207

Type

Journal article

Journal

Acta Crystallogr D Biol Crystallogr

Publication Date

01/2006

Volume

62

Pages

32 - 47

Keywords

Cryoprotective Agents, Crystallization, Crystallography, X-Ray, Freezing, Macromolecular Substances, Protein Conformation, Proteins