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We describe a user-friendly immunohistochemical approach for the detection of protein localization in Drosophila ovaries, here focusing on CTP synthase. This approach mainly uses fluorescently labeled antibodies to detect single, double, or multiple antigens. We provide a step-by-step protocol with detailed notes and tips, a simplified method that can also be adapted to detect protein localization beyond Drosophila ovaries.

Original publication

DOI

10.1007/978-1-4939-2851-4_13

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2015

Volume

1328

Pages

179 - 189

Keywords

Animals, Antibodies, Carbon-Nitrogen Ligases, Drosophila melanogaster, Female, Immunohistochemistry, Ovarian Follicle