Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Most pathologies of the brain have an inflammatory component, associated with the release of cytokines such as interleukin-1beta (IL-1beta) from resident and infiltrating cells. The IL-1 type I receptor (IL-1RI) initiates a signalling cascade but the type II receptor (IL-1RII) acts as a decoy receptor. Here we have investigated the expression of IL-1beta, IL-1RI and IL-1RII in distinct inflammatory lesions in the rat brain. IL-1beta was injected into the brain to generate an inflammatory lesion in the absence of neuronal cell death whereas neuronal death was specifically induced by the microinjection of N-methyl-D-aspartate (NMDA). Using TaqMan RT-PCR and ELISA, we observed elevated de novo IL-1beta synthesis 2 h after the intracerebral microinjection of IL-1beta; this de novo IL-1beta remained elevated 24 h later. There was a concomitant increase in IL-1RI mRNA but a much greater increase in IL-1RII mRNA. Immunostaining revealed that IL-1RII was expressed on brain endothelial cells and on infiltrating neutrophils. In contrast, although IL-1beta and IL-1RI were elevated to similar levels in response to NMDA challenge, the response was delayed and IL-1RII mRNA expression was unchanged. The lesion-specific expression of IL-1 receptors suggests that the receptors are differentially regulated in a manner not directly related to the endogenous level of IL-1 in the CNS.

Original publication

DOI

10.1111/j.1460-9568.2005.03965.x

Type

Journal article

Journal

Eur J Neurosci

Publication Date

03/2005

Volume

21

Pages

1205 - 1214

Keywords

Animals, Blotting, Western, Chemokines, CXC, Encephalitis, Enzyme-Linked Immunosorbent Assay, Gene Expression Regulation, Immunohistochemistry, Immunoprecipitation, Intercellular Signaling Peptides and Proteins, Interleukin-1, Male, N-Methylaspartate, RNA, Messenger, Rats, Rats, Wistar, Receptors, Interleukin-1, Receptors, Interleukin-1 Type I, Receptors, Interleukin-1 Type II, Reverse Transcriptase Polymerase Chain Reaction, Statistics, Nonparametric, Time Factors