Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

In Saccharomyces cerevisiae, the Nrd1-Nab3-Sen1 pathway mediates the termination of snoRNAs and cryptic unstable transcripts (CUTs). Both Nrd1 and the Set1 histone H3K4 methyltransferase complex interact with RNA polymerase II (Pol II) during early elongation, leading us to test whether these two processes are functionally linked. The deletion of SET1 exacerbates the growth rate and termination defects of nrd1 mutants. Set1 is important for the appropriate recruitment of Nrd1. Additionally, Set1 modulates histone acetylation levels in the promoter-proximal region via the Rpd3L deacetylase and NuA3 acetyltransferase complexes, both of which contain PHD finger proteins that bind methylated H3K4. Increased levels of histone acetylation reduce the efficiency of Nrd1-dependent termination. We speculate that Set1 promotes proper early termination by the Nrd1-Nab3-Sen1 complex by affecting the kinetics of Pol II transcription in early elongation.

Original publication

DOI

10.1128/MCB.05590-11

Type

Journal article

Journal

Mol Cell Biol

Publication Date

09/2011

Volume

31

Pages

3569 - 3583

Keywords

Acetylation, Blotting, Northern, Chromatin Immunoprecipitation, DNA Helicases, Histone Deacetylases, Histone-Lysine N-Methyltransferase, Histones, Lysine, Methylation, Mutation, Nuclear Proteins, Plasmids, Protein Binding, RNA Helicases, RNA Polymerase II, RNA, Small Nucleolar, RNA-Binding Proteins, Reverse Transcriptase Polymerase Chain Reaction, Saccharomyces cerevisiae Proteins, Signal Transduction, Transcription, Genetic