Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Ion channel gating may be studied by recording the electrical current flowing through a single ion channel in response to a periodically varied linear voltage ramp. We describe a novel computational procedure for the analysis of such data, based upon the construction of single-channel current-voltage surfaces. Analysis of current-voltage surfaces may be used to measure single channel conductances, and to demonstrate voltage dependent channel gating. We verify the procedure using computer simulated data for a simple model of a voltage-gated ion channel. The method is illustrated by its application to data from ion channels formed in planar bilayers by two peptides: (a) des-Ile1, Asn2-mastoparan; and (b) δ-toxin from Staphylococcus aureus. © 1990 Academic Press Limited.

Original publication

DOI

10.1016/S0022-5193(05)80321-2

Type

Journal article

Journal

Journal of Theoretical Biology

Publication Date

14/05/1990

Volume

144

Pages

213 - 223