Solution structures of the two PBZ domains from human APLF and their interaction with poly(ADP-ribose).
Eustermann S., Brockmann C., Mehrotra PV., Yang J-C., Loakes D., West SC., Ahel I., Neuhaus D.
Addition of poly(ADP-ribose) (PAR) is an important post-translational modification in higher eukaryotes. Several DNA repair and checkpoint proteins possess specific PAR-binding zinc-finger (PBZ) modules critical for function. Here, we present solution structures of the two PBZ modules of aprataxin and PNK-like factor (APLF), revealing a novel type of zinc finger. By combining in vivo PAR-binding data with NMR interaction data using PAR fragments, we propose a structural basis for PBZ-PAR recognition.