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A detailed understanding of the interaction between the highly variant Plasmodium falciparum erythrocyte membrane proteins 1 (PfEMP1) and their human binding partners is essential to explain their roles in disease development in malaria, as well as to understand how antibodies can inhibit these interactions and how the parasite manages to evade such an immune response. This chapter focuses on using surface plasmon resonance (SPR) as a reproducible, high-throughput method to quantitatively characterize these interactions. We describe how to utilize protein A or A/G and streptavidin for protein immobilization on SPR sensor chips and provide instructions on how to biotinylate proteins for this purpose and how to use SPR for binding competition assays. Since these experiments rely on recombinant proteins, we also present a method to verify their structural integrity using circular dichroism spectroscopy.

Original publication

DOI

10.1007/978-1-0716-2189-9_35

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2022

Volume

2470

Pages

467 - 482

Keywords

Antibodies, Binding assay, Circular dichroism spectroscopy, Malaria, PfEMP1, Surface plasmon resonance, Antibodies, Protozoan, Carrier Proteins, Erythrocytes, Humans, Malaria, Falciparum, Plasmodium falciparum, Protozoan Proteins, Recombinant Proteins, Surface Plasmon Resonance