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Despite their clinical importance, detailed analysis of ligand binding at G-protein coupled receptors (GPCRs) has proved difficult. Here we successfully measure the binding of a GPCR, neurotensin receptor-1 (NTS-1), to its ligand, neurotensin (NT), using surface plasmon resonance (SPR). Specific responses were observed between NT and purified, detergent-solublised, recombinant NTS-1, using a novel configuration where the biotinylated NT ligand was immobilised on the biosensor surface. This SPR approach shows promise as a generic approach for the study of ligand interactions with other suitable GPCRs.

Original publication

DOI

10.1007/s00249-006-0070-x

Type

Journal article

Journal

Eur Biophys J

Publication Date

10/2006

Volume

35

Pages

709 - 712

Keywords

Animals, Kinetics, Ligands, Neurotensin, Rats, Receptors, Neurotensin, Surface Plasmon Resonance